Construct: shRNA TRCN0000100115

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018825.2-2459s1c1
DNA Barcode:
CCACTCCCATTAGCAGCTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sh2b2 (23921)
Gene Description:
SH2B adaptor protein 2
Transcript:
RefSeq NM_018825.2 (NON-CURRENT)
Match location:
Position 2459 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000100115)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACTCCCATTAGCAGCTATT
Hairpin Sequence:
5'-CCGG-CCACTCCCATTAGCAGCTATT-CTCGAG-AATAGCTGCTAATGGGAGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACTCCCATTAGCAGCTATTCTCGAGAATAGCTGCTAATGGGAGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACTCCCATTAGCAGCTATTCTCGAGAATAGCTGCTAATGGGAGTGG-3'

Other clones with same target sequence:

(none)