Construct: shRNA TRCN0000100276

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008168.1-1575s1c1
DNA Barcode:
CCTGGAGAACCCGTATGTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Grik5 (14809)
Gene Description:
glutamate receptor, ionotropic, kainate 5 (gamma 2)
Transcript:
RefSeq NM_008168.1 (NON-CURRENT)
Match location:
Position 1575 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000100276)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGGAGAACCCGTATGTTAT
Hairpin Sequence:
5'-CCGG-CCTGGAGAACCCGTATGTTAT-CTCGAG-ATAACATACGGGTTCTCCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGGAGAACCCGTATGTTATCTCGAGATAACATACGGGTTCTCCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGGAGAACCCGTATGTTATCTCGAGATAACATACGGGTTCTCCAGG-3'

Other clones with same target sequence:

(none)