Construct: shRNA TRCN0000100309

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146072.1-1874s1c1
DNA Barcode:
GACCAGAATAGTTGGAGGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Grik1 (14805)
Gene Description:
glutamate receptor, ionotropic, kainate 1
Transcript:
RefSeq NM_146072.1 (NON-CURRENT)
Match location:
Position 1874 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000100309)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACCAGAATAGTTGGAGGAAT
Hairpin Sequence:
5'-CCGG-GACCAGAATAGTTGGAGGAAT-CTCGAG-ATTCCTCCAACTATTCTGGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACCAGAATAGTTGGAGGAATCTCGAGATTCCTCCAACTATTCTGGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACCAGAATAGTTGGAGGAATCTCGAGATTCCTCCAACTATTCTGGTC-3'

Other clones with same target sequence:

(none)