Construct: shRNA TRCN0000100358

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025859.1-672s1c1
DNA Barcode:
GCAATGGAATGGTTAGTTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Arl1 (104303)
Gene Description:
ADP-ribosylation factor-like 1
Transcript:
RefSeq NM_025859.1 (NON-CURRENT)
Match location:
Position 672 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000100358)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAATGGAATGGTTAGTTGAA
Hairpin Sequence:
5'-CCGG-GCAATGGAATGGTTAGTTGAA-CTCGAG-TTCAACTAACCATTCCATTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAATGGAATGGTTAGTTGAACTCGAGTTCAACTAACCATTCCATTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAATGGAATGGTTAGTTGAACTCGAGTTCAACTAACCATTCCATTGC-3'

Other clones with same target sequence:

  1. TRCN0000287554