Construct: shRNA TRCN0000100371

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007476.2-156s1c1
DNA Barcode:
GCAGGGAAGACAACAATTCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Arf1 (11840)
Gene Description:
ADP-ribosylation factor 1
Transcript:
RefSeq NM_007476.2 (NON-CURRENT)
Match location:
Position 156 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000100371)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGGGAAGACAACAATTCTA
Hairpin Sequence:
5'-CCGG-GCAGGGAAGACAACAATTCTA-CTCGAG-TAGAATTGTTGTCTTCCCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGGGAAGACAACAATTCTACTCGAGTAGAATTGTTGTCTTCCCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGGGAAGACAACAATTCTACTCGAGTAGAATTGTTGTCTTCCCTGC-3'

Other clones with same target sequence:

  1. TRCN0000324832