Construct: shRNA TRCN0000100378

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133365.1-4318s1c1
DNA Barcode:
CCAGAATCAATTCGATAACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dnah5 (110082)
Gene Description:
dynein, axonemal, heavy chain 5
Transcript:
RefSeq NM_133365.1 (NON-CURRENT)
Match location:
Position 4318 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000100378)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGAATCAATTCGATAACAT
Hairpin Sequence:
5'-CCGG-CCAGAATCAATTCGATAACAT-CTCGAG-ATGTTATCGAATTGATTCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGAATCAATTCGATAACATCTCGAGATGTTATCGAATTGATTCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGAATCAATTCGATAACATCTCGAGATGTTATCGAATTGATTCTGG-3'

Other clones with same target sequence:

(none)