Construct: shRNA TRCN0000100401

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029494.2-719s1c1
DNA Barcode:
CAGCTATTTGACTTGTTGTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rab30 (75985)
Gene Description:
RAB30, member RAS oncogene family
Transcript:
RefSeq NM_029494.2 (CURRENT)
Match location:
Position 719 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000100401)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGCTATTTGACTTGTTGTAA
Hairpin Sequence:
5'-CCGG-CAGCTATTTGACTTGTTGTAA-CTCGAG-TTACAACAAGTCAAATAGCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGCTATTTGACTTGTTGTAACTCGAGTTACAACAAGTCAAATAGCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGCTATTTGACTTGTTGTAACTCGAGTTACAACAAGTCAAATAGCTG-3'

Other clones with same target sequence:

(none)