Construct: shRNA TRCN0000100411

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001025358.1-1093s1c1
DNA Barcode:
CCTGATGTTGCCAAACAGTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Klc1 (16593)
Gene Description:
kinesin light chain 1
Transcript:
RefSeq NM_001025358.1 (NON-CURRENT)
Match location:
Position 1093 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000100411)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGATGTTGCCAAACAGTTA
Hairpin Sequence:
5'-CCGG-CCTGATGTTGCCAAACAGTTA-CTCGAG-TAACTGTTTGGCAACATCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGATGTTGCCAAACAGTTACTCGAGTAACTGTTTGGCAACATCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGATGTTGCCAAACAGTTACTCGAGTAACTGTTTGGCAACATCAGG-3'

Other clones with same target sequence:

(none)