Construct: shRNA TRCN0000100443

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009680.2-726s1c1
DNA Barcode:
CCCGGATAGAATCGATCTGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ap3b1 (11774)
Gene Description:
adaptor-related protein complex 3, beta 1 subunit
Transcript:
RefSeq NM_009680.2 (NON-CURRENT)
Match location:
Position 726 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000100443)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCGGATAGAATCGATCTGAT
Hairpin Sequence:
5'-CCGG-CCCGGATAGAATCGATCTGAT-CTCGAG-ATCAGATCGATTCTATCCGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCGGATAGAATCGATCTGATCTCGAGATCAGATCGATTCTATCCGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCGGATAGAATCGATCTGATCTCGAGATCAGATCGATTCTATCCGGG-3'

Other clones with same target sequence:

(none)