Construct: shRNA TRCN0000100465

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011256.1-6365s1c1
DNA Barcode:
GCCTTCTCTTTGATTTCTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pitpnm2 (19679)
Gene Description:
phosphatidylinositol transfer protein, membrane-associated 2
Transcript:
RefSeq NM_011256.1 (NON-CURRENT)
Match location:
Position 6365 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000100465)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTTCTCTTTGATTTCTAAA
Hairpin Sequence:
5'-CCGG-GCCTTCTCTTTGATTTCTAAA-CTCGAG-TTTAGAAATCAAAGAGAAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTTCTCTTTGATTTCTAAACTCGAGTTTAGAAATCAAAGAGAAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTTCTCTTTGATTTCTAAACTCGAGTTTAGAAATCAAAGAGAAGGC-3'

Other clones with same target sequence:

(none)