Construct: shRNA TRCN0000100529

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175255.2-1180s1c1
DNA Barcode:
GCGGCTCATAATACTTATGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sec24a (77371)
Gene Description:
Sec24 related gene family, member A (S. cerevisiae)
Transcript:
RefSeq NM_175255.2 (NON-CURRENT)
Match location:
Position 1180 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000100529)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGGCTCATAATACTTATGAT
Hairpin Sequence:
5'-CCGG-GCGGCTCATAATACTTATGAT-CTCGAG-ATCATAAGTATTATGAGCCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGGCTCATAATACTTATGATCTCGAGATCATAAGTATTATGAGCCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGGCTCATAATACTTATGATCTCGAGATCATAAGTATTATGAGCCGC-3'

Other clones with same target sequence:

(none)