Construct: shRNA TRCN0000100542

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023854.1-463s1c1
DNA Barcode:
CCAGAGAAGAAAGACTCTGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Arfgap2 (77038)
Gene Description:
ADP-ribosylation factor GTPase activating protein 2
Transcript:
RefSeq NM_023854.1 (NON-CURRENT)
Match location:
Position 463 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000100542)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGAGAAGAAAGACTCTGAT
Hairpin Sequence:
5'-CCGG-CCAGAGAAGAAAGACTCTGAT-CTCGAG-ATCAGAGTCTTTCTTCTCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGAGAAGAAAGACTCTGATCTCGAGATCAGAGTCTTTCTTCTCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGAGAAGAAAGACTCTGATCTCGAGATCAGAGTCTTTCTTCTCTGG-3'

Other clones with same target sequence:

(none)