Construct: shRNA TRCN0000100550

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007454.1-3732s1c1
DNA Barcode:
GCACATTTCTTCCCTCTACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ap1b1 (11764)
Gene Description:
adaptor protein complex AP-1, beta 1 subunit
Transcript:
RefSeq NM_007454.1 (NON-CURRENT)
Match location:
Position 3732 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000100550)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACATTTCTTCCCTCTACAA
Hairpin Sequence:
5'-CCGG-GCACATTTCTTCCCTCTACAA-CTCGAG-TTGTAGAGGGAAGAAATGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACATTTCTTCCCTCTACAACTCGAGTTGTAGAGGGAAGAAATGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACATTTCTTCCCTCTACAACTCGAGTTGTAGAGGGAAGAAATGTGC-3'

Other clones with same target sequence:

(none)