Construct: shRNA TRCN0000100555

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001008498.1-1609s1c1
DNA Barcode:
AGCACTGACTACTCTTCTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Wdr34 (71820)
Gene Description:
WD repeat domain 34
Transcript:
RefSeq NM_001008498.1 (NON-CURRENT)
Match location:
Position 1609 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000100555)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCACTGACTACTCTTCTGAA
Hairpin Sequence:
5'-CCGG-AGCACTGACTACTCTTCTGAA-CTCGAG-TTCAGAAGAGTAGTCAGTGCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCACTGACTACTCTTCTGAACTCGAGTTCAGAAGAGTAGTCAGTGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCACTGACTACTCTTCTGAACTCGAGTTCAGAAGAGTAGTCAGTGCT-3'

Other clones with same target sequence:

(none)