Construct: shRNA TRCN0000100588

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019640.2-176s1c1
DNA Barcode:
GTCCTGAAGAATGAACCTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pitpnb (56305)
Gene Description:
phosphatidylinositol transfer protein, beta
Transcript:
RefSeq NM_019640.2 (NON-CURRENT)
Match location:
Position 176 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000100588)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCCTGAAGAATGAACCTTAT
Hairpin Sequence:
5'-CCGG-GTCCTGAAGAATGAACCTTAT-CTCGAG-ATAAGGTTCATTCTTCAGGAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCCTGAAGAATGAACCTTATCTCGAGATAAGGTTCATTCTTCAGGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCCTGAAGAATGAACCTTATCTCGAGATAAGGTTCATTCTTCAGGAC-3'

Other clones with same target sequence:

  1. TRCN0000334288