Construct: shRNA TRCN0000100618

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028721.2-3766s1c1
DNA Barcode:
GCTGTTGAAATTCGGCAGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nphp3 (74025)
Gene Description:
nephronophthisis 3 (adolescent)
Transcript:
RefSeq NM_028721.2 (NON-CURRENT)
Match location:
Position 3766 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000100618)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGTTGAAATTCGGCAGAAA
Hairpin Sequence:
5'-CCGG-GCTGTTGAAATTCGGCAGAAA-CTCGAG-TTTCTGCCGAATTTCAACAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGTTGAAATTCGGCAGAAACTCGAGTTTCTGCCGAATTTCAACAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGTTGAAATTCGGCAGAAACTCGAGTTTCTGCCGAATTTCAACAGC-3'

Other clones with same target sequence:

(none)