Construct: shRNA TRCN0000100621

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177411.2-359s1c1
DNA Barcode:
CCGTGTGTTTAGATGACACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rab5b (19344)
Gene Description:
RAB5B, member RAS oncogene family
Transcript:
RefSeq NM_177411.2 (NON-CURRENT)
Match location:
Position 359 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000100621)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGTGTGTTTAGATGACACAA
Hairpin Sequence:
5'-CCGG-CCGTGTGTTTAGATGACACAA-CTCGAG-TTGTGTCATCTAAACACACGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGTGTGTTTAGATGACACAACTCGAGTTGTGTCATCTAAACACACGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGTGTGTTTAGATGACACAACTCGAGTTGTGTCATCTAAACACACGG-3'

Other clones with same target sequence:

(none)