Construct: shRNA TRCN0000100656

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145509.1-336s1c1
DNA Barcode:
CCGCTCAATGGTATCAACATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rab7b (226421)
Gene Description:
RAB7B, member RAS oncogene family
Transcript:
RefSeq NM_145509.1 (NON-CURRENT)
Match location:
Position 336 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000100656)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGCTCAATGGTATCAACATT
Hairpin Sequence:
5'-CCGG-CCGCTCAATGGTATCAACATT-CTCGAG-AATGTTGATACCATTGAGCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGCTCAATGGTATCAACATTCTCGAGAATGTTGATACCATTGAGCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGCTCAATGGTATCAACATTCTCGAGAATGTTGATACCATTGAGCGG-3'

Other clones with same target sequence:

(none)