Construct: shRNA TRCN0000100681

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019817.1-49s1c1
DNA Barcode:
GCCATCCTGATTCTGGACAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Copz1 (56447)
Gene Description:
coatomer protein complex, subunit zeta 1
Transcript:
RefSeq NM_019817.1 (CURRENT)
Match location:
Position 49 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000100681)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCATCCTGATTCTGGACAAT
Hairpin Sequence:
5'-CCGG-GCCATCCTGATTCTGGACAAT-CTCGAG-ATTGTCCAGAATCAGGATGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCATCCTGATTCTGGACAATCTCGAGATTGTCCAGAATCAGGATGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCATCCTGATTCTGGACAATCTCGAGATTGTCCAGAATCAGGATGGC-3'

Other clones with same target sequence:

  1. TRCN0000363778