Construct: shRNA TRCN0000100758

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016810.2-351s1c1
DNA Barcode:
CAGGATTATACACACGAATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gosr1 (53334)
Gene Description:
golgi SNAP receptor complex member 1
Transcript:
RefSeq NM_016810.2 (NON-CURRENT)
Match location:
Position 351 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000100758)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGGATTATACACACGAATTT
Hairpin Sequence:
5'-CCGG-CAGGATTATACACACGAATTT-CTCGAG-AAATTCGTGTGTATAATCCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGGATTATACACACGAATTTCTCGAGAAATTCGTGTGTATAATCCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGGATTATACACACGAATTTCTCGAGAAATTCGTGTGTATAATCCTG-3'

Other clones with same target sequence:

  1. TRCN0000323678