Construct: shRNA TRCN0000100768

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025921.1-260s1c1
DNA Barcode:
GCAATACAGAAGTTGCAAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
2610002M06Rik (67028)
Gene Description:
RIKEN cDNA 2610002M06 gene
Transcript:
RefSeq NM_025921.1 (NON-CURRENT)
Match location:
Position 260 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000100768)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAATACAGAAGTTGCAAGAA
Hairpin Sequence:
5'-CCGG-GCAATACAGAAGTTGCAAGAA-CTCGAG-TTCTTGCAACTTCTGTATTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAATACAGAAGTTGCAAGAACTCGAGTTCTTGCAACTTCTGTATTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAATACAGAAGTTGCAAGAACTCGAGTTCTTGCAACTTCTGTATTGC-3'

Other clones with same target sequence:

  1. TRCN0000332128