Construct: shRNA TRCN0000100778

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025445.2-1245s1c1
DNA Barcode:
TGCCGATTCCTACTGGAAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Arfgap3 (66251)
Gene Description:
ADP-ribosylation factor GTPase activating protein 3
Transcript:
RefSeq NM_025445.2 (NON-CURRENT)
Match location:
Position 1245 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000100778)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCCGATTCCTACTGGAAGAA
Hairpin Sequence:
5'-CCGG-TGCCGATTCCTACTGGAAGAA-CTCGAG-TTCTTCCAGTAGGAATCGGCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCCGATTCCTACTGGAAGAACTCGAGTTCTTCCAGTAGGAATCGGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCCGATTCCTACTGGAAGAACTCGAGTTCTTCCAGTAGGAATCGGCA-3'

Other clones with same target sequence:

(none)