Construct: shRNA TRCN0000100783

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_147612.3-582s1c1
DNA Barcode:
CCTTGTGATAGATAGCACAGA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Arl5c (217151)
Gene Description:
ADP-ribosylation factor-like 5C
Transcript:
RefSeq XM_147612.3 (NON-CURRENT)
Match location:
Position 582 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000100783)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTGTGATAGATAGCACAGA
Hairpin Sequence:
5'-CCGG-CCTTGTGATAGATAGCACAGA-CTCGAG-TCTGTGCTATCTATCACAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTGTGATAGATAGCACAGACTCGAGTCTGTGCTATCTATCACAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTGTGATAGATAGCACAGACTCGAGTCTGTGCTATCTATCACAAGG-3'

Other clones with same target sequence:

(none)