Construct: shRNA TRCN0000100832

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024436.1-593s1c1
DNA Barcode:
GCAGGGAACAAGTGCGATCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rab22a (19334)
Gene Description:
RAB22A, member RAS oncogene family
Transcript:
RefSeq NM_024436.1 (NON-CURRENT)
Match location:
Position 593 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000100832)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGGGAACAAGTGCGATCTT
Hairpin Sequence:
5'-CCGG-GCAGGGAACAAGTGCGATCTT-CTCGAG-AAGATCGCACTTGTTCCCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGGGAACAAGTGCGATCTTCTCGAGAAGATCGCACTTGTTCCCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGGGAACAAGTGCGATCTTCTCGAGAAGATCGCACTTGTTCCCTGC-3'

Other clones with same target sequence:

  1. TRCN0000302790