Construct: shRNA TRCN0000100861

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008996.2-581s1c1
DNA Barcode:
GCCGAGAAGTCCAATGTTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rab1a (19324)
Gene Description:
RAB1A, member RAS oncogene family
Transcript:
RefSeq NM_008996.2 (NON-CURRENT)
Match location:
Position 581 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000100861)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCGAGAAGTCCAATGTTAAA
Hairpin Sequence:
5'-CCGG-GCCGAGAAGTCCAATGTTAAA-CTCGAG-TTTAACATTGGACTTCTCGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGAGAAGTCCAATGTTAAACTCGAGTTTAACATTGGACTTCTCGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGAGAAGTCCAATGTTAAACTCGAGTTTAACATTGGACTTCTCGGC-3'

Other clones with same target sequence:

(none)