Construct: shRNA TRCN0000100865
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_010863.1-3537s1c1
- DNA Barcode:
- GCTAACTGTTAGCGTCCTCTT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Myo1b (17912)
- Gene Description:
- myosin IB
- Transcript:
- RefSeq NM_010863.1 (NON-CURRENT)
- Match location:
- Position 3537 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000100865)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCTAACTGTTAGCGTCCTCTT
- Hairpin Sequence:
-
5'-CCGG-GCTAACTGTTAGCGTCCTCTT-CTCGAG-AAGAGGACGCTAACAGTTAGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCTAACTGTTAGCGTCCTCTTCTCGAGAAGAGGACGCTAACAGTTAGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCTAACTGTTAGCGTCCTCTTCTCGAGAAGAGGACGCTAACAGTTAGC-3'
Other clones with same target sequence:
-
TRCN0000302721
