Construct: shRNA TRCN0000100867

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010863.1-1166s1c1
DNA Barcode:
GCGTTCAGTTTCCGAACAGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Myo1b (17912)
Gene Description:
myosin IB
Transcript:
RefSeq NM_010863.1 (NON-CURRENT)
Match location:
Position 1166 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000100867)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGTTCAGTTTCCGAACAGTT
Hairpin Sequence:
5'-CCGG-GCGTTCAGTTTCCGAACAGTT-CTCGAG-AACTGTTCGGAAACTGAACGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGTTCAGTTTCCGAACAGTTCTCGAGAACTGTTCGGAAACTGAACGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGTTCAGTTTCCGAACAGTTCTCGAGAACTGTTCGGAAACTGAACGC-3'

Other clones with same target sequence:

  1. TRCN0000302795