Construct: shRNA TRCN0000100894

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009147.2-675s1c1
DNA Barcode:
CCTACAGCTTTGGTTGGACTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sec23a (20334)
Gene Description:
SEC23 homolog A, COPII coat complex component
Transcript:
RefSeq NM_009147.2 (CURRENT)
Match location:
Position 675 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000100894)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTACAGCTTTGGTTGGACTT
Hairpin Sequence:
5'-CCGG-CCTACAGCTTTGGTTGGACTT-CTCGAG-AAGTCCAACCAAAGCTGTAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTACAGCTTTGGTTGGACTTCTCGAGAAGTCCAACCAAAGCTGTAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTACAGCTTTGGTTGGACTTCTCGAGAAGTCCAACCAAAGCTGTAGG-3'

Other clones with same target sequence:

(none)