Construct: shRNA TRCN0000100927

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019718.2-562s1c1
DNA Barcode:
CTGCAAGAATGTCAACGCAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Arl3 (56350)
Gene Description:
ADP-ribosylation factor-like 3
Transcript:
RefSeq NM_019718.2 (CURRENT)
Match location:
Position 562 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000100927)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGCAAGAATGTCAACGCAAA
Hairpin Sequence:
5'-CCGG-CTGCAAGAATGTCAACGCAAA-CTCGAG-TTTGCGTTGACATTCTTGCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGCAAGAATGTCAACGCAAACTCGAGTTTGCGTTGACATTCTTGCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGCAAGAATGTCAACGCAAACTCGAGTTTGCGTTGACATTCTTGCAG-3'

Other clones with same target sequence:

(none)