Construct: shRNA TRCN0000100950

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177824.2-1277s1c1
DNA Barcode:
CCAGTCTTAATCTTCTGGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
9830107B12Rik (328829)
Gene Description:
RIKEN cDNA 9830107B12 gene
Transcript:
RefSeq NM_177824.2 (NON-CURRENT)
Match location:
Position 1277 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000100950)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGTCTTAATCTTCTGGAAT
Hairpin Sequence:
5'-CCGG-CCAGTCTTAATCTTCTGGAAT-CTCGAG-ATTCCAGAAGATTAAGACTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGTCTTAATCTTCTGGAATCTCGAGATTCCAGAAGATTAAGACTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGTCTTAATCTTCTGGAATCTCGAGATTCCAGAAGATTAAGACTGG-3'

Other clones with same target sequence:

(none)