Construct: shRNA TRCN0000101068

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145827.2-3031s1c1
DNA Barcode:
CCCGGACTGTAAACTACAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nlrp3 (216799)
Gene Description:
NLR family, pyrin domain containing 3
Transcript:
RefSeq NM_145827.2 (NON-CURRENT)
Match location:
Position 3031 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000101068)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCGGACTGTAAACTACAGAT
Hairpin Sequence:
5'-CCGG-CCCGGACTGTAAACTACAGAT-CTCGAG-ATCTGTAGTTTACAGTCCGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCGGACTGTAAACTACAGATCTCGAGATCTGTAGTTTACAGTCCGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCGGACTGTAAACTACAGATCTCGAGATCTGTAGTTTACAGTCCGGG-3'

Other clones with same target sequence:

(none)