Construct: shRNA TRCN0000101113

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008376.3-276s1c1
DNA Barcode:
CGGCAAGAGTGCCACTGGCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gimap1 (16205)
Gene Description:
GTPase, IMAP family member 1
Transcript:
RefSeq NM_008376.3 (CURRENT)
Match location:
Position 276 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000101113)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGCAAGAGTGCCACTGGCAA
Hairpin Sequence:
5'-CCGG-CGGCAAGAGTGCCACTGGCAA-CTCGAG-TTGCCAGTGGCACTCTTGCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGCAAGAGTGCCACTGGCAACTCGAGTTGCCAGTGGCACTCTTGCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGCAAGAGTGCCACTGGCAACTCGAGTTGCCAGTGGCACTCTTGCCG-3'

Other clones with same target sequence:

(none)