Construct: shRNA TRCN0000101137

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021286.2-2590s1c1
DNA Barcode:
CCCAAGTGTCTCTTGGAACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sez6 (20370)
Gene Description:
seizure related gene 6
Transcript:
RefSeq NM_021286.2 (NON-CURRENT)
Match location:
Position 2590 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000101137)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAAGTGTCTCTTGGAACAA
Hairpin Sequence:
5'-CCGG-CCCAAGTGTCTCTTGGAACAA-CTCGAG-TTGTTCCAAGAGACACTTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAAGTGTCTCTTGGAACAACTCGAGTTGTTCCAAGAGACACTTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAAGTGTCTCTTGGAACAACTCGAGTTGTTCCAAGAGACACTTGGG-3'

Other clones with same target sequence:

(none)