Construct: shRNA TRCN0000101165

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007652.2-1422s1c1
DNA Barcode:
CCCGCCTCTAAATAAATAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cd59a (12509)
Gene Description:
CD59a antigen
Transcript:
RefSeq NM_007652.2 (NON-CURRENT)
Match location:
Position 1422 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000101165)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCGCCTCTAAATAAATAAAT
Hairpin Sequence:
5'-CCGG-CCCGCCTCTAAATAAATAAAT-CTCGAG-ATTTATTTATTTAGAGGCGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCGCCTCTAAATAAATAAATCTCGAGATTTATTTATTTAGAGGCGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCGCCTCTAAATAAATAAATCTCGAGATTTATTTATTTAGAGGCGGG-3'

Other clones with same target sequence:

(none)