Construct: shRNA TRCN0000101170

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172807.1-2178s1c1
DNA Barcode:
GCACTAAATTGAGGCAAATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ppwd1 (238831)
Gene Description:
peptidylprolyl isomerase domain and WD repeat containing 1
Transcript:
RefSeq NM_172807.1 (NON-CURRENT)
Match location:
Position 2178 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000101170)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACTAAATTGAGGCAAATAA
Hairpin Sequence:
5'-CCGG-GCACTAAATTGAGGCAAATAA-CTCGAG-TTATTTGCCTCAATTTAGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACTAAATTGAGGCAAATAACTCGAGTTATTTGCCTCAATTTAGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACTAAATTGAGGCAAATAACTCGAGTTATTTGCCTCAATTTAGTGC-3'

Other clones with same target sequence:

(none)