Construct: shRNA TRCN0000101262

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028450.1-273s1c1
DNA Barcode:
GCCAGCTTCATAGAATATCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gulp1 (70676)
Gene Description:
GULP, engulfment adaptor PTB domain containing 1
Transcript:
RefSeq NM_028450.1 (NON-CURRENT)
Match location:
Position 273 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000101262)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAGCTTCATAGAATATCAT
Hairpin Sequence:
5'-CCGG-GCCAGCTTCATAGAATATCAT-CTCGAG-ATGATATTCTATGAAGCTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAGCTTCATAGAATATCATCTCGAGATGATATTCTATGAAGCTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAGCTTCATAGAATATCATCTCGAGATGATATTCTATGAAGCTGGC-3'

Other clones with same target sequence:

(none)