Construct: shRNA TRCN0000101270

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029803.1-239s1c1
DNA Barcode:
CTCCACATCAACAAATGCCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ifi27l2a (76933)
Gene Description:
interferon, alpha-inducible protein 27 like 2A
Transcript:
RefSeq NM_029803.1 (NON-CURRENT)
Match location:
Position 239 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000101270)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCCACATCAACAAATGCCAT
Hairpin Sequence:
5'-CCGG-CTCCACATCAACAAATGCCAT-CTCGAG-ATGGCATTTGTTGATGTGGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCCACATCAACAAATGCCATCTCGAGATGGCATTTGTTGATGTGGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCCACATCAACAAATGCCATCTCGAGATGGCATTTGTTGATGTGGAG-3'

Other clones with same target sequence:

(none)