Construct: shRNA TRCN0000101277

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021524.1-1127s1c1
DNA Barcode:
GCCACCTTATCTTAGAGTCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nampt (59027)
Gene Description:
nicotinamide phosphoribosyltransferase
Transcript:
RefSeq NM_021524.1 (NON-CURRENT)
Match location:
Position 1127 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000101277)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCACCTTATCTTAGAGTCAT
Hairpin Sequence:
5'-CCGG-GCCACCTTATCTTAGAGTCAT-CTCGAG-ATGACTCTAAGATAAGGTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCACCTTATCTTAGAGTCATCTCGAGATGACTCTAAGATAAGGTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCACCTTATCTTAGAGTCATCTCGAGATGACTCTAAGATAAGGTGGC-3'

Other clones with same target sequence:

(none)