Construct: shRNA TRCN0000101282

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027351.1-532s1c1
DNA Barcode:
CCACACTTGGACATGAAATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ppil3 (70225)
Gene Description:
peptidylprolyl isomerase (cyclophilin)-like 3
Transcript:
RefSeq NM_027351.1 (NON-CURRENT)
Match location:
Position 532 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000101282)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACACTTGGACATGAAATAT
Hairpin Sequence:
5'-CCGG-CCACACTTGGACATGAAATAT-CTCGAG-ATATTTCATGTCCAAGTGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACACTTGGACATGAAATATCTCGAGATATTTCATGTCCAAGTGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACACTTGGACATGAAATATCTCGAGATATTTCATGTCCAAGTGTGG-3'

Other clones with same target sequence:

(none)