Construct: shRNA TRCN0000101330

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016751.2-2173s1c1
DNA Barcode:
CCTGTCATTCATCTTGCTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Clec4f (51811)
Gene Description:
C-type lectin domain family 4, member f
Transcript:
RefSeq NM_016751.2 (NON-CURRENT)
Match location:
Position 2173 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000101330)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGTCATTCATCTTGCTAAT
Hairpin Sequence:
5'-CCGG-CCTGTCATTCATCTTGCTAAT-CTCGAG-ATTAGCAAGATGAATGACAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGTCATTCATCTTGCTAATCTCGAGATTAGCAAGATGAATGACAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGTCATTCATCTTGCTAATCTCGAGATTAGCAAGATGAATGACAGG-3'

Other clones with same target sequence:

(none)