Construct: shRNA TRCN0000101347

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023680.2-323s1c1
DNA Barcode:
CTGTGATAAAGATCAGGAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tnfrsf22 (79202)
Gene Description:
tumor necrosis factor receptor superfamily, member 22
Transcript:
RefSeq NM_023680.2 (NON-CURRENT)
Match location:
Position 323 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000101347)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGTGATAAAGATCAGGAAAT
Hairpin Sequence:
5'-CCGG-CTGTGATAAAGATCAGGAAAT-CTCGAG-ATTTCCTGATCTTTATCACAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGTGATAAAGATCAGGAAATCTCGAGATTTCCTGATCTTTATCACAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGTGATAAAGATCAGGAAATCTCGAGATTTCCTGATCTTTATCACAG-3'

Other clones with same target sequence:

(none)