Construct: shRNA TRCN0000101375

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011240.2-9324s1c1
DNA Barcode:
GCAGTTGAATTGATGCGTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ranbp2 (19386)
Gene Description:
RAN binding protein 2
Transcript:
RefSeq NM_011240.2 (NON-CURRENT)
Match location:
Position 9324 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000101375)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTTGAATTGATGCGTAAT
Hairpin Sequence:
5'-CCGG-GCAGTTGAATTGATGCGTAAT-CTCGAG-ATTACGCATCAATTCAACTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTTGAATTGATGCGTAATCTCGAGATTACGCATCAATTCAACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTTGAATTGATGCGTAATCTCGAGATTACGCATCAATTCAACTGC-3'

Other clones with same target sequence:

  1. TRCN0000326929