Construct: shRNA TRCN0000101392

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175406.1-374s1c1
DNA Barcode:
GCAGCTATATGATAGACAATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Atp6v0d2 (242341)
Gene Description:
ATPase, H+ transporting, lysosomal V0 subunit D2
Transcript:
RefSeq NM_175406.1 (NON-CURRENT)
Match location:
Position 374 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000101392)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGCTATATGATAGACAATA
Hairpin Sequence:
5'-CCGG-GCAGCTATATGATAGACAATA-CTCGAG-TATTGTCTATCATATAGCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGCTATATGATAGACAATACTCGAGTATTGTCTATCATATAGCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGCTATATGATAGACAATACTCGAGTATTGTCTATCATATAGCTGC-3'

Other clones with same target sequence:

(none)