Construct: shRNA TRCN0000101414

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011889.1-1495s1c1
DNA Barcode:
GCTTTCTCCATGACTCTCCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sept3 (24050)
Gene Description:
septin 3
Transcript:
RefSeq NM_011889.1 (NON-CURRENT)
Match location:
Position 1495 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000101414)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTTCTCCATGACTCTCCAT
Hairpin Sequence:
5'-CCGG-GCTTTCTCCATGACTCTCCAT-CTCGAG-ATGGAGAGTCATGGAGAAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTTCTCCATGACTCTCCATCTCGAGATGGAGAGTCATGGAGAAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTTCTCCATGACTCTCCATCTCGAGATGGAGAGTCATGGAGAAAGC-3'

Other clones with same target sequence:

(none)