Construct: shRNA TRCN0000101415

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009190.1-2630s1c1
DNA Barcode:
CCTGCAAACAATTACTAGGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Vps4b (20479)
Gene Description:
vacuolar protein sorting 4B
Transcript:
RefSeq NM_009190.1 (NON-CURRENT)
Match location:
Position 2630 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000101415)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGCAAACAATTACTAGGTT
Hairpin Sequence:
5'-CCGG-CCTGCAAACAATTACTAGGTT-CTCGAG-AACCTAGTAATTGTTTGCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGCAAACAATTACTAGGTTCTCGAGAACCTAGTAATTGTTTGCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGCAAACAATTACTAGGTTCTCGAGAACCTAGTAATTGTTTGCAGG-3'

Other clones with same target sequence:

(none)