Construct: shRNA TRCN0000101416

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009190.1-1340s1c1
DNA Barcode:
GCTGATCCTAACTGCATTGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Vps4b (20479)
Gene Description:
vacuolar protein sorting 4B
Transcript:
RefSeq NM_009190.1 (NON-CURRENT)
Match location:
Position 1340 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000101416)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGATCCTAACTGCATTGTA
Hairpin Sequence:
5'-CCGG-GCTGATCCTAACTGCATTGTA-CTCGAG-TACAATGCAGTTAGGATCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGATCCTAACTGCATTGTACTCGAGTACAATGCAGTTAGGATCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGATCCTAACTGCATTGTACTCGAGTACAATGCAGTTAGGATCAGC-3'

Other clones with same target sequence:

  1. TRCN0000354161