Construct: shRNA TRCN0000101424

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_053156.1-422s1c1
DNA Barcode:
CGACGTGGACATTTCTTACTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Allc (94041)
Gene Description:
allantoicase
Transcript:
RefSeq NM_053156.1 (NON-CURRENT)
Match location:
Position 422 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000101424)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGACGTGGACATTTCTTACTT
Hairpin Sequence:
5'-CCGG-CGACGTGGACATTTCTTACTT-CTCGAG-AAGTAAGAAATGTCCACGTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGACGTGGACATTTCTTACTTCTCGAGAAGTAAGAAATGTCCACGTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGACGTGGACATTTCTTACTTCTCGAGAAGTAAGAAATGTCCACGTCG-3'

Other clones with same target sequence:

(none)