Construct: shRNA TRCN0000101425

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175025.2-3535s1c1
DNA Barcode:
GCTTTCTTACTAACACACAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Atp2c1 (235574)
Gene Description:
ATPase, Ca++-sequestering
Transcript:
RefSeq NM_175025.2 (NON-CURRENT)
Match location:
Position 3535 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000101425)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTTCTTACTAACACACAAA
Hairpin Sequence:
5'-CCGG-GCTTTCTTACTAACACACAAA-CTCGAG-TTTGTGTGTTAGTAAGAAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTTCTTACTAACACACAAACTCGAGTTTGTGTGTTAGTAAGAAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTTCTTACTAACACACAAACTCGAGTTTGTGTGTTAGTAAGAAAGC-3'

Other clones with same target sequence:

(none)