Construct: shRNA TRCN0000101471

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019734.1-1046s1c1
DNA Barcode:
CCGGCCAAGAAGTGTCTAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Asah1 (11886)
Gene Description:
N-acylsphingosine amidohydrolase 1
Transcript:
RefSeq NM_019734.1 (NON-CURRENT)
Match location:
Position 1046 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000101471)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGGCCAAGAAGTGTCTAAAT
Hairpin Sequence:
5'-CCGG-CCGGCCAAGAAGTGTCTAAAT-CTCGAG-ATTTAGACACTTCTTGGCCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGGCCAAGAAGTGTCTAAATCTCGAGATTTAGACACTTCTTGGCCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGGCCAAGAAGTGTCTAAATCTCGAGATTTAGACACTTCTTGGCCGG-3'

Other clones with same target sequence:

(none)