Construct: shRNA TRCN0000101503

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023179.2-559s1c1
DNA Barcode:
CAACTATCGGGTTACTGTCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Atp6v1g2 (66237)
Gene Description:
ATPase, H+ transporting, lysosomal V1 subunit G2
Transcript:
RefSeq NM_023179.2 (NON-CURRENT)
Match location:
Position 559 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000101503)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAACTATCGGGTTACTGTCTA
Hairpin Sequence:
5'-CCGG-CAACTATCGGGTTACTGTCTA-CTCGAG-TAGACAGTAACCCGATAGTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAACTATCGGGTTACTGTCTACTCGAGTAGACAGTAACCCGATAGTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAACTATCGGGTTACTGTCTACTCGAGTAGACAGTAACCCGATAGTTG-3'

Other clones with same target sequence:

(none)